Updates#

2026-07-14#

• MFEprimer v4.3.1 (same version, updated binaries): *.spec.tsv now includes productTm and ta (same values as the text report’s ProductTm / Ta). Re-download from GitHub Releases if you need these columns.

2025-11-13 13:19:09#

• New database add for MP-Ref and Spec: Taihu goose T2T genome (GCF_040182565.1)

2025-09-29 23:28:19#

• MFEprimer command line version 4.2.4 released with a running bug fixed.

2025-09-18 19:04:00#

• Add new app: MP-Methy: Multiplex primer design for bisulfite sequencing and methylation-specific PCR, facilitating research in DNA methylation and epigenetics.

2025-08-22 18:04:43#

• Add new app: Retrieve subsequence from FASTA.

2025-07-22 10:23:59#

• MP-Ref fix: prevent bdb1/bdb2 being same as db.
• MP-Ref fix: correct primer coverage condition in sangerDesign.
• MP-Ref doc: add SMN1 repeat region guide and update documentation.

2025-07-21 21:54:57#

• MP-Ref is online: Multiplex PCR Primer Design for Reference Genomes. The old name is MP2.

2024-07-18 16:27:51#

• Improve candidate primer selection strategy for large amplicons, e.g., 5000 bp, to save memory and time.

2024-06-13 17:51:40#

• Add two new databases for spec and mp2: Anser cygnoides domesticus (swan goose) and Tremarctos ornatus (spectacled bear).